Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice
Identifieur interne : 002550 ( Main/Exploration ); précédent : 002549; suivant : 002551Effect of mucosal and systemic immunization with virus-like particles of severe acute respiratory syndrome coronavirus in mice
Auteurs : Baojing Lu [République populaire de Chine] ; Yi Huang ; Li Huang ; Bao Li [République populaire de Chine] ; Zhenhua Zheng ; Ze Chen ; Jianjun Chen ; Qinxue Hu ; Hanzhong WangSource :
- Immunology [ 0019-2805 ] ; 2010.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques (pharmacologie), Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (sang), Anticorps neutralisants (immunologie), Anticorps neutralisants (sang), Baculoviridae, Femelle, Immunisation (), Immunité muqueuse (), Immunité muqueuse (immunologie), Immunoglobuline A (immunologie), Immunoglobuline A (sang), Immunoglobuline G (immunologie), Immunoglobuline G (sang), Modèles animaux de maladie humaine, Oligodésoxyribonucléotides (pharmacocinétique), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (sang), Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Vaccins antiviraux (pharmacocinétique), Virus du SRAS (génétique), Virus du SRAS (immunologie).
- MESH :
- génétique : Vaccins antiviraux, Virus du SRAS.
- immunologie : Anticorps antiviraux, Anticorps neutralisants, Immunité muqueuse, Immunoglobuline A, Immunoglobuline G, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Virus du SRAS.
- pharmacocinétique : Oligodésoxyribonucléotides, Vaccins antiviraux.
- pharmacologie : Adjuvants immunologiques.
- sang : Anticorps antiviraux, Anticorps neutralisants, Immunoglobuline A, Immunoglobuline G, Syndrome respiratoire aigu sévère.
- Animaux, Baculoviridae, Femelle, Immunisation, Immunité muqueuse, Modèles animaux de maladie humaine, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (pharmacology), Animals, Antibodies, Neutralizing (blood), Antibodies, Neutralizing (immunology), Antibodies, Viral (blood), Antibodies, Viral (immunology), Baculoviridae, Disease Models, Animal, Female, Immunity, Mucosal (drug effects), Immunity, Mucosal (immunology), Immunization (methods), Immunoglobulin A (blood), Immunoglobulin A (immunology), Immunoglobulin G (blood), Immunoglobulin G (immunology), Mice, Mice, Inbred BALB C, Oligodeoxyribonucleotides (pharmacokinetics), SARS Virus (genetics), SARS Virus (immunology), Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Viral Vaccines (genetics), Viral Vaccines (immunology), Viral Vaccines (pharmacokinetics).
- MESH :
- chemical , blood : Antibodies, Neutralizing, Antibodies, Viral, Immunoglobulin A, Immunoglobulin G.
- chemical , genetics : Viral Vaccines.
- chemical , immunology : Antibodies, Neutralizing, Antibodies, Viral, Immunoglobulin A, Immunoglobulin G, Viral Vaccines.
- chemical , pharmacokinetics : Oligodeoxyribonucleotides, Viral Vaccines.
- chemical , pharmacology : Adjuvants, Immunologic.
- blood : Severe Acute Respiratory Syndrome.
- drug effects : Immunity, Mucosal.
- genetics : SARS Virus.
- immunology : Immunity, Mucosal, SARS Virus, Severe Acute Respiratory Syndrome.
- methods : Immunization.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Baculoviridae, Disease Models, Animal, Female, Mice, Mice, Inbred BALB C.
Abstract
Nasal administration has emerged as a promising and attractive route for vaccination, especially for the prophylaxis of respiratory diseases. Our previous studies have shown that severe acute respiratory syndrome coronavirus (SARS-CoV) virus-like particles (VLPs) can be assembled using a recombinant baculovirus (rBV) expression system and such VLPs induce specific humoral and cellular immune responses in mice after subcutaneous injection. Here, we investigated mucosal immune responses to SARS-CoV VLPs in a mouse model. Mice were immunized in parallel, intraperitoneally or intranasally, with VLPs alone or with VLPs plus cytosine–phosphate–guanosine (CpG). Immune responses, including the production of SARS-CoV-specific serum immunoglobulin G (IgG) and secretory immunoglobulin A (sIgA), were determined in mucosal secretions and tissues. Both immunizations induced SARS-CoV-specific IgG, although the levels of IgG in groups immunized via the intraperitoneal (i.p.) route were higher. sIgA was detected in saliva in groups immunized intranasally but not in groups immunized intraperitoneally. CpG had an adjuvant effect on IgA production in genital tract washes when administered intranasally but only affected IgA production in faeces samples when administered intraperitoneally. In addition, IgA was also detected in mucosal tissues from the lung and intestine, while CpG induced an increased level of IgA in the intestine. Most importantly, neutralization antibodies were detected in sera after i.p. and intranasal (i.n.) immunizations. Secretions in genital tract washes from the i.n. group also showed neutralization activity. Furthermore, VLPs that were administered intraperitoneally elicited cellular immune responses as demonstrated by enzyme-linked immunospot (ELISPOT) assay analyses. In summary, our study indicates that mucosal immunization with rBV SARS-CoV VLPs represent an effective means for eliciting protective systemic and mucosal immune responses against SARS-CoV, providing important information for vaccine design.
Url:
DOI: 10.1111/j.1365-2567.2010.03231.x
PubMed: 20406307
PubMed Central: 2878469
Affiliations:
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Le document en format XML
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<term>Antibodies, Neutralizing (immunology)</term>
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<term>Immunity, Mucosal (drug effects)</term>
<term>Immunity, Mucosal (immunology)</term>
<term>Immunization (methods)</term>
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<term>Immunoglobulin G (immunology)</term>
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<term>Severe Acute Respiratory Syndrome (immunology)</term>
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<front><div type="abstract" xml:lang="en"><p>Nasal administration has emerged as a promising and attractive route for vaccination, especially for the prophylaxis of respiratory diseases. Our previous studies have shown that severe acute respiratory syndrome coronavirus (SARS-CoV) virus-like particles (VLPs) can be assembled using a recombinant baculovirus (rBV) expression system and such VLPs induce specific humoral and cellular immune responses in mice after subcutaneous injection. Here, we investigated mucosal immune responses to SARS-CoV VLPs in a mouse model. Mice were immunized in parallel, intraperitoneally or intranasally, with VLPs alone or with VLPs plus cytosine–phosphate–guanosine (CpG). Immune responses, including the production of SARS-CoV-specific serum immunoglobulin G (IgG) and secretory immunoglobulin A (sIgA), were determined in mucosal secretions and tissues. Both immunizations induced SARS-CoV-specific IgG, although the levels of IgG in groups immunized via the intraperitoneal (i.p.) route were higher. sIgA was detected in saliva in groups immunized intranasally but not in groups immunized intraperitoneally. CpG had an adjuvant effect on IgA production in genital tract washes when administered intranasally but only affected IgA production in faeces samples when administered intraperitoneally. In addition, IgA was also detected in mucosal tissues from the lung and intestine, while CpG induced an increased level of IgA in the intestine. Most importantly, neutralization antibodies were detected in sera after i.p. and intranasal (i.n.) immunizations. Secretions in genital tract washes from the i.n. group also showed neutralization activity. Furthermore, VLPs that were administered intraperitoneally elicited cellular immune responses as demonstrated by enzyme-linked immunospot (ELISPOT) assay analyses. In summary, our study indicates that mucosal immunization with rBV SARS-CoV VLPs represent an effective means for eliciting protective systemic and mucosal immune responses against SARS-CoV, providing important information for vaccine design.</p>
</div>
</front>
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<name sortKey="Hu, Qinxue" sort="Hu, Qinxue" uniqKey="Hu Q" first="Qinxue" last="Hu">Qinxue Hu</name>
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<name sortKey="Huang, Yi" sort="Huang, Yi" uniqKey="Huang Y" first="Yi" last="Huang">Yi Huang</name>
<name sortKey="Wang, Hanzhong" sort="Wang, Hanzhong" uniqKey="Wang H" first="Hanzhong" last="Wang">Hanzhong Wang</name>
<name sortKey="Zheng, Zhenhua" sort="Zheng, Zhenhua" uniqKey="Zheng Z" first="Zhenhua" last="Zheng">Zhenhua Zheng</name>
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